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Plant Breeding

Basics of Genetics

Basics of Genetics

· Incomplete dominance in dihybrids – 3:6:3:1:2:1. Eg: Mirabilis jalapa. Complementory factors (Duplicate recessive epistatis) 9:7, Test cross ratio 1:3. Two separate factors governed the same character. Supplementory factors (Recessive epistasis) – 9:3:4, Test cross ratio 1:1:2. Epistatic factor (dominant epistaris) – 12:3:1. Test cross ratio 2:1:1. The gene which …

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Sexual and Asexual Plant Propagation

Sexual and ASexual Plant Propagation

Sexual and Asexual Plant Propagation Sexual and Asexual Plant Propagation: Unlike agronomic plants, many horticultural plants are troublesome for seed propagation methods. Horticultural plants have annual to perennial life cycle and seed propagation is not economical. In such cases vegetative propagation is more effective. It is done by using various plant parts for …

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Southern Northern and Western Hybridization

Southern Northern and Western Hybridization

Nucleic acid hybridization is a basic technique in molecular biology which takes advantage of the ability of individual single-stranded nucleic acid molecules to form double-stranded molecules. According to Watson-Crick base pairing, adenine binds with thymine and guanine binds with cytosine by hydrogen bonding. Southern hybridization: The basic principle behind the …

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construction and uses of genomic and cDNA Libraries

genomic and cDNA Libraries

A genomic library contains all the sequences present in the genome of an organism (apart from any sequences, such as telomeres that cannot be readily cloned). It is a collection of cloned, restriction-enzyme-digested DNA frag­ments containing at least one copy of every DNA sequence in a genome. The entire genome …

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